Optical Stimulation and Imaging of Functional Brain Circuitry in a Laminar Flow Chamber
نویسندگان
چکیده
We report a microfluidic platform for the photostimulation and functional imaging of living brain slices. Fast voltage sensitive dye imaging and stimulation techniques combined with microfluidic chambers were used to study spontaneous and stimulated neuronal activities in the neonatal mouse hippocampus. Caged glutamate was locally delivered in specific regions of interest, while laser uncaging of glutamate initiated neural signaling. Both stimulated and spontaneous neural activity displayed similar spatiotemporal characteristics. In addition, through local calcium depletion in segmented flow, our data suggest that stimulated neural activity in the developing hippocampus is dependent on synaptic signaling rather than direct neural coupling.
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